Immunomodulating Effects of Tolpa Peat Preparation

Abstract:

3 tests were used in the study of the effects of Tolpa Peat Preparation (TPP) on the immune system and the immune response, i.e. the tests of: angiogenesis, migration, and spontaneous release of IL-1 through its effect on the formation of thermostable E-rosettes. Mononuclear leukocytes for the tests were obtained from peripheral blood and articular fluid of patients with rheumatoid arthritis. The effect of TPP on angiogenesis in the local reaction of GvH type was studied in Balb/c mice. In a dose of 50 mg/kg, TPP had no effect on angiogenesis stimulated with mononuclear leukocytes of healthy humans, but it inhibited increased angiogenesis stimulated by these cells from the peripheral blood and articular fluid of rheumatoid arthritis patients. At lower doses, TPP increased the angiogenesis, i.e. the stimulating activity of mononuclear leukocytes, of healthy humans. The effect of TPP on the migration of these leukocytes was tested in direct and indirect migration tests. TPP inhibited this migration during direct contact with the migrating cells, but failed to increase the release from these cells of factors stimulating or inhibiting this migration. The supernatants of cultures of mononuclear leukocytes showed a high activity of IL-1 in most rheumatoid arthritis cases. The release of this activity in cultures was inhibited by TPP in concentrations of 100 pg/ml. These results confirm the supposition that TPP influences the activity of the immune-response cells and further clinical trials of the preparation are certainly desirable.

Authors:

Skopinska-Rozewska, E. et al.

Abbreviated Journal Title: Reumatologia
Date of Publication: 1991
Journal Volume: XXIX
Page Numbers: 161 through 171
Country of Publication: Poland
Language of Article: Polish, English translation available
Issue/Part/Supplement: 2
ISSN:

MESH Headings:

Tolpa Peat Preparation
GvH Reaction
Interleukins
Migration
Mononuclear Leukocytes
Rheumatoid Arthritis

Published with permission from Reumatologia.