The Dose-Dependent Effect of Tolpa Peat Preparation on the Humoral Response of Mice Immunized with Sheep Erythrocytes

Abstract:

The Balb/c mice were immunized by a single intraperitoneal administration of 0.2 cm3 of 10% sheep erythrocytes suspension (SRBC). Tolpa Peat Preparation (TPP) that was used in the study was a peat-derived substance obtained in the laboratory of Professor Stanislaw Tolpa. It was given to the mice intraperitoneally, 4 times at 24-hour intervals. The first dose was administered 2 hours before immunization. Testing was performed with the following doses: 0.5 , 2.5, 5, 10, 25, 50, 100, and 250 mg/kg. The assays included the number of spleen cells producing anti-SRBC hemolisins, the level of serum hemagglutinins to SRBC (19S+7S types), and the percentage of splenocytes forming spontaneous E-rosettes.

It was found that the effect of TPP on these immunologic parameters of the mice immunized with SRBC was dose-dependent. The effect potentiating the humoral response occurred for the doses of 0.5 - 10 mg/kg. The doses of 25 and 50 mg/kg did not affect the immunologic response to SRBC, while the doses of 100 and 250 mg/kg had a suppressive effect. The impact of TPP on the percentage of splenocytes forming E-rosettes was also dose dependent, but in this case stimulating activity was observed for the doses of 2.5-25 mg/kg.

Authors:

Obminska-Domoradzka, B.
Switala, M.
Debowy, J.
Garbulinski, T.

Abbreviated Journal Title: Acta Pol Pharm
Date of Publication: 1993
Journal Volume: 50
Page Numbers: 497 through 500
Country of Publication: Poland
Language of Article: English
Issue/Part/Supplement: 4-5
ISSN: 0001-6837

MESH Headings:

Peat Preparation
Splenocytes
Immunotropic Activity

Published with permission from Acta Poloniae Pharmaceutica - Drug Research.