The Effect of Tolpa Peat Preparation (TPP) on Human Mononuclear Leukocyte-Induced Angiogenesis

Abstract:

TPP enhanced the ability of nonactivated human mononuclear leukocytes (MNC) derived from the blood of young and elderly donors, patients with coronary artery disease and with oral candidiasis, to induce neovascularization in the local graft versus host (GvH) reaction (H-LIA assay), and diminished the high activity of lymphocytes from rheumatoid arthritic patients, but did not influence the response of MNC in diabetic patients with proliferative retinopathy. Various fractions of mononuclear leukocytes were grafted intradermally into immunosuppressed (with cyclophosphamide) Balb/c mice. Recipients were then treated with TPP or saline solution. After 3 days, the local GvH reaction was evaluated as the mean number of newly formed blood vessels surrounding the cell graft sites. CD4+ and CD8+ theophylline sensitive Fc_Y+ lymphocytes and monocytes responded to TPP through a significant enhancement of their angiogenic activity.

Authors:

Skopinska-Rozewska, E.
Polakowski, I.
Zukowska, M.
Radomska, D.
Malkowska-Zwierz, W.
Demkow, V.
Skopinski, P.
Pazdur, J.
Liszewska-Pfeifer, D.

Abbreviated Journal Title: Herba Pol
Date of Publication: 1993
Journal Volume: XXXIX
Page Numbers: 41 through 51
Country of Publication: Poland
Language of Article: English
Issue/Part/Supplement: 1-2
ISSN: 0018-0599

MESH Headings:

Tolpa Peat Preparation
GvH Reaction
Angiogenic Activity
Mononuclear Leukocytes

Published with permission from Herba Polonica