Production of Cytokines by Mouse Peritoneal Cells Treated with Tolpa Peat Preparation (TPP): Dependence on Age and Strain of the Mice

Abstract:

The purpose of the study was to investigate the production of two cytokines, i.e. Interferon beta (IFN-ß) and tumor necrosis factor alpha (TNF-a) by freshly isolated resident mouse peritoneal cells (RPC) treated with the immunostimulating drug Tolpa Peat Preparation (TPP). Nontoxic concentrations of TPP (10 and 100 µg/ml) potentiated the cytokine production by RPC in BALB/c mice. The effects were dose-dependent. The optimal cytokine-inducing concentration of TPP was 100 µg/ml. The effect of TPP depended on the age of the mouse. High potentiation (16-fold) of IFN-ß and TNF-a production by TPP was observed when 5- and 8-week-old mice were used. However, in RPC isolated from one-year-old mice, only two (fold) potentiation of IFN and TNF was observed. The activity of selected commercial batches of TPP was compared to RPC cultures isolated from three different strains of mice: BALB/c, C3H/HeJ, and NZB. A significant stimulation of IFN and TNF (1-3 fold) was observed in the cells of NZB mice. Peritoneal cells of C3H mice did not respond to stimulation with TPP.

Authors:

Blach-Olszewska, Z.
Zaczynska, E.
Broniarek, E.
Inglot, A.D.

Abbreviated Journal Title: Arch Immun Ther Exp
Date of Publication: 1993
Journal Volume: 41
Page Numbers: 81 through 85
Country of Publication: Poland
Language of Article: English
Issue/Part/Supplement:
ISSN: 0004-069X

MESH Headings:

Tolpa Peat Preparation
Cytokine Production
Mouse Resident Peritoneal Cells
Interferons
Tumor Necrosis Factor
Immunomodulation

Published with permission from Archivum Immunologiae et Therapiae Experimentalis