A Method to Assess the Immunomodulating Effects of Tolpa Peat Preparation (TPP) by Measuring the Hyporeactivity to Interferon Induction and the Tumor Necrosis Factor Response

Abstract:

Tolpa Peat Preparation (TPP) is an immunomodulator which was found to be an inducer of interferon (IFN) and tumor necrosis factor (TNF) in human peripheral blood leukocytes (PBL). PBL cultures prepared from the blood of healthy volunteers taking a 5 mg TPP tablet daily lose the ability to respond to IFN induction by TPP, phytohemagglutinin (PHA) or lipopolysaccharide (LPS). The phenomenon of hyporeactivity or tolerance is characteristic of every IFN inducer described to date. In the TPP-treated volunteers the tolerance developed over the course of three weeks and lasted one to two weeks. In comparison with the tolerance to the IFN induction, the hyporeactivity to TNF induction developed slowly and was only partial. Because the detection of tolerance to IFN induction is a very sensitive indicator of TPP activity in vivo in patients taking the drug orally, we suggest that it may be applied to assess the mode of action, dosage and schedule of administration of the IFN-inducing immunomodulator.

Author:

Inglot, A.D.
Zielinska-Jenczylik, J.
Sypula, A.

Abbreviated Journal Title: Arch Imm Ther Exp
Date of Publication: 1993
Journal Volume: 41
Page Numbers: 87 through 93
Country of Publication: Poland
Language of Article: English
Issue/Part/Supplement: 4-5
ISSN: 0004-069X

MESH Headings:

Tolpa Peat Preparation
Immunomodulation
Interferon Induction
Tumor Necrosis Factor Induction
Tolerance to Interferon Induction in Humans
Blood Leukocytes

Published with permission from Archivum Immunologiae et Therapiae Experimentalis