Influence of Long-Term Administration of Tolpa Peat Preparation on Immune Reactivity in Mice. II. The Effect of Intermittent TPP Administration on the Morphological Picture of Lymphatic Organs
Abstract:
The studies were conducted on 200 8-week-old Balb/c mice (inbred strain). The mice received Tolpa Peat Preparation (TPP) in drinking water (10 or 50 mg/kg/day) for 7 weeks (experiment I). Two lobes of the thymus, the spleen, and the axillary and mesenteric lymph nodes of the mice were taken for histological examinations after 3, 5 and 7 weeks of continuous TPP treatment, and also 12 and 16 weeks after the beginning of the treatment. In experiment II, TPP was administered daily at the same doses for 3 weeks and intermittently for 9 consecutive weeks; a one-week interval followed each seven-day cycle. The lymphatic organs of the mice were taken for histological examinations after 3, 5, 7, 9, and 12 weeks of TPP treatment.
It was found that daily administration of TPP (as described above) to mice for 7 weeks (10 mg/kg) induced a marked stimulation of the lymphatic organs (in the first weeks of TPP treatment) and resulted in retrograde changes (depletion of thymocytes and lymphocytes) in the lymphatic structures when TPP administration was prolonged (4-7 weeks).
The morphological changes due to TPP administration in the dose of 50 mg/kg were less pronounced in the organs that were examined. The retrograde changes in the lymphatic structures disappeared 9 weeks after TPP treatment had stopped. Similarly, the changes showing stimulation of the lymphatic organs were maintained throughout the entire 9-week period. A reduction of the daily administration of TPP to 3 weeks, which was followed by 9 weeks of treatment every other week for 7 consecutive days, prevented the occurrence of retrograde morphological changes in the lymphatic organs, but its stimulating effect was weaker than that observed in the case of continuous administration.
Authors:
Madej, J.A.
Obminska-Domoradzka, B.
Garbulinski, T.
Abbreviated Journal Title: Acta Pol Pharm
Date of Publication: 1993
Journal Volume: 50
Page Numbers: 405 through 408
Country of Publication: Poland
Language of Article: English
Issue/Part/Supplement: 4-5
ISSN: 0001-6837
MESH Headings:
Peat Preparation
Lymphatic Organs
Morphological Changes
Published with permission from Acta Poloniae Pharmaceutica - Drug Research