Restoration of Cyclophosphamide-Suppressed Primary Immune Response in Mice by Tolpa Peat Preparation (TPP). A Comparison with Calf Thymus Extract

Abstract:

In order to induce pharmacological immunosuppression in Balb/c mice, the animals were given 200 mg/kg of cyclophosphamide intraperitoneally 144 hours or 24 hours prior to SRBC immunization. Tolpa Peat Preparation (TPP) was administered in doses of 10 and 50 mg/kg on the day of immunization and then every 24 hours for 3 consecutive days. It was given to the cyclophosphamide-treated and non-treated mice. Calf thymus extract (TFX) was administered (1 and 5 mg/kg) simultaneously, for comparison with TPP. The following immunological indices were determined: the percentage of splenocytes forming E- and EAC-rosettes, the number of plaque-forming cells (PFC), and the levels of 19S+7S and 7S anti-SRBC hemagglutinins.

The immunosuppressing action of a single cyclophosphamide dose decreased the percentage of EAC rosettes, the number of PFC and serum anti-SRBC hemagglutinin titer. TPP is able to restore the percentage of EAC rosettes, the number of PFC and anti-SRBC hemagglutinin titer, either partially or totally, after cyclophosphamide-induced immunosuppression. The effectiveness of TPP depends mainly on the dose and timing of cyclophosphamide administration prior to SRBC immunization. The most pronounced restoration of the cells in mice was observed with the TPP dose of 50 mg/kg after cyclophosphamide suppression 144 h before immunization. TFX (1 mg/kg) only partially restored the number of PFC and 7S anti-SRBC hemagglutinin titer, which had been depressed due to cyclophosphamide treatment 144 h prior to immunization. However, it did not change the depressing effect of cyclophosphamide on the percentage of EAC rosette-forming splenocytes. In the mice that were treated with cyclophosphamide 24 h prior to immunization, TFX-Polfa did not change the suppressing action of cyclophosphamide, but was able to stimulate the E-rosette-forming splenocytes.

Author:

Obminska-Domoradzka, B.

Abbreviated Journal Title: Acta Pol Pharm
Date of Publication: 1993
Journal Volume: 50
Page Numbers: 501 through 506
Country of Publication: Poland
Language of Article: English
Issue/Part/Supplement: 6
ISSN: 0001-6837

MESH Headings:

Peat Preparation
Immunosuppression

Published with permission from Acta Poloniae Pharmaceutica - Drug Research