Investigations of Allergenic Properties of Tolpa Peat Preparation
Abstract:
The ability of Tolpa Peat Preparation (TPP) to induce or enhance an allergic sensitization was tested on mice and guinea pigs. The levels of IgE antibody in mouse sera and IgG1 as well as IgE antibody levels in guinea pig sera were evaluated by PCA (Passive Cutaneous Anaphylaxis) tests. When adsorbed on aluminum hydroxide gel (alum) and introduced into BALB/c mice by several subcutaneous injections, TPP was unable to stimulate a noticeable anti-TPP IgE antibody response. When introduced together with ovalbumin (OA) into the mice in the course of immunization with OA, TPP did not enhance anti-OA antibody response. TPP adsorbed on alum and injected subcutaneously into guinea pigs was unable to induce noticeable IgG1a, IgG1b, or IgE antibody response, and mast cells obtained from the lungs and mesenteries of these animals did not release histamine when challenged with TPP in vitro at 37oC. In conclusion, our results show that under the experimental conditions used in the present experiments TPP was unable to induce or enhance an allergic sensitization in mice and guinea pigs.
Author:
Maslinski, Cz.
Wyczolkowska, J.
Czuwaj, M.
Michon, T.
Abbreviated Journal Title: Acta Pol Pharm
Date of Publication: 1993
Journal Volume: 50
Page Numbers: 469 through 474
Country of Publication: Poland
Language of Article: English
Issue/Part/Supplement: 6
ISSN: 0001-6837
MESH Headings:
Peat Preparation
Immunization
Published with permission from Acta Poloniae Pharmaceutica - Drug Research